Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma.

ABSTRACT:

Background

Extracellular vesicles (EVs), known as cell-derived membranous structures harboring a variety of biomolecules, have been widely used in liquid biopsy. Due to the complex biological composition of plasma, plasma RNA omics analysis (RNomics) is easily affected, thus it is necessary to select an optimal strategy from exiting methods according to the performance for intended application.

Methods

In this study, four different strategies for EVs isolation were performed and compared (i.e. ultracentrifugation (UC), size exclusion chromatography (SEC), and two most frequently-used commercially available isolation kit (ExoQuick and exoEasy). We compared the yield, purity, PCR quantification of RNAs, miRNA-seq analyses and mRNA-seq analyses of RNAs from EVs isolated using f

SUBMITTER: Yang Y 

PROVIDER: S-EPMC7953782 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature