Resveratrol induces PD-L1 expression through snail-driven activation of Wnt pathway in lung cancer cells.

ABSTRACT:

Purpose

Recent clinical trials with agents targeting immune checkpoint pathway have emerged as an important therapeutic approach for a broad range of cancer types. Resveratrol has been shown to possess cancer preventive and therapeutic effects and has potential to be chemotherapeutic agent/adjuvant. Here, we assessed the effect of resveratrol on immune checkpoint pathways.

Methods

The expression patterns of Wnt components and PD-L1 were examined by Western blot, Chromatin immunoprecipitation (ChIP) was used for analysis of DNA-protein interaction, the promoter activity was determined by luciferase reporter assay, apoptosis was analyzed by flow cytometry and the ability of the resveratrol to modulate T cell function was assessed in a co-culture system.

Results

Although

SUBMITTER: Yang M 

PROVIDER: S-EPMC7954741 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature