Characterization of Plasmid Co-Harboring NDM-1 and SHV-12 from a Multidrug-Resistant Citrobacter freundii Strain ZT01-0079 in China.

ABSTRACT:

Background

The emergence of multidrug-resistant Citrobacter freundii poses daunting challenges to the treatment of clinical infections. The purpose of this study was to characterize the genome of a C. freundii strain with an IncX3 plasmid encoding both the bla _NDM-1 and bla _SHV-12 genes.

Methods

Strain ZT01-0079 was isolated from a clinical urine sample. The Vitek2 system was used for identification and antimicrobial susceptibility testing. The presence of bla _NDM-1 was detected by PCR and sequencing. Conjugation experiments and Southern blotting were performed to determine the transferability of the bla _NDM-1- carrying plasmid. Nanopore and Illumina sequencing were performed to better unde

SUBMITTER: Zhang T 

PROVIDER: S-EPMC7955740 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature