Complexity and graded regulation of neuronal cell-type-specific alternative splicing revealed by single-cell RNA sequencing.

ABSTRACT: The enormous cellular diversity in the mammalian brain, which is highly prototypical and organized in a hierarchical manner, is dictated by cell-type-specific gene-regulatory programs at the molecular level. Although prevalent in the brain, the contribution of alternative splicing (AS) to the molecular diversity across neuronal cell types is just starting to emerge. Here, we systematically investigated AS regulation across over 100 transcriptomically defined neuronal types of the adult mouse cortex using deep single-cell RNA-sequencing data. We found distinct splicing programs between glutamatergic and GABAergic neurons and between subclasses within each neuronal class. These programs consist of overlapping sets of alternative exons showing differential splicing at multiple hierarchical le

SUBMITTER: Feng H 

PROVIDER: S-EPMC7958184 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature