N6-methyladenosine modification of HCV RNA genome regulates cap-independent IRES-mediated translation via YTHDC2 recognition.

ABSTRACT: Hepatitis C virus (HCV) infections are associated with the risk of progression to fibrosis, cirrhosis, and hepatocellular carcinoma. The HCV RNA genome is translated by an internal ribosome entry site (IRES)-dependent mechanism. The structure and function of the HCV IRES have been investigated by both biological and biophysical criteria. Recently, the role of N6-methyladenosine (m⁶A) in cellular RNA and viral transcripts has been intensely investigated. The HCV RNA genome is m⁶A-methylated, and this modification regulates the viral life cycle. In this study, we investigated the role of m⁶A modification of the HCV genome in the IRES-dependent translation function by mutating m⁶A consensus motifs (DRACH) within the IRES element in stem-loop III and

SUBMITTER: Kim GW 

PROVIDER: S-EPMC7958429 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature