DNA Polymerase II Supports the Replicative Bypass of N²-Alkyl-2'-deoxyguanosine Lesions in Escherichia coli Cells.

ABSTRACT: Alkylation represents a main form of DNA damage. The N² position of guanine is frequently alkylated in DNA. The SOS-induced polymerases have been shown to be capable of bypassing various DNA damage products in Escherichia coli. Herein, we explored the influences of four N²-alkyl-dG lesions (alkyl = ethyl, n-butyl, isobutyl, or sec-butyl) on DNA replication in AB1157 E. coli cells and the corresponding strains with polymerases (Pol) II, IV, and V being individually or simultaneously knocked out. We found that N²-Et-dG is slightly less blocking to DNA replication than the N²-Bu-dG lesions, which display very similar replication bypass efficiencies. Additionally, Pol II and, to a lesser degree,

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7965357 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature