Circ_0003266 sponges miR-503-5p to suppress colorectal cancer progression via regulating PDCD4 expression.

ABSTRACT:

Background

Circular RNAs (circRNAs) feature prominently in tumor progression. However, the biological function and molecular mechanism of circ_0003266 in colorectal cancer (CRC) require further investigation.

Methods

Circ_0003266 expression in 46 pairs CRC tissues / adjacent tissues, and CRC cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR); after circ_0003266 was overexpressed or knocked down in CRC cells, cell proliferation, apoptosis, migration, and invasion were evaluated by the cell counting kit-8 (CCK-8), flow cytometry, and Transwell assays, respectively; the interaction among circ_0003266, miR-503-5p, and programmed cell death 4 (PDCD4) was confirmed using bioinformatics analysis and dual-luciferase reporter assay; PDCD4 protein ex

SUBMITTER: Wen C 

PROVIDER: S-EPMC7968268 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature