Gold nanoparticle-based immunochromatographic assay for detection Pseudomonas aeruginosa in water and food samples.

ABSTRACT: Pseudomonas aeruginosa (P. aeruginosa) is the common infection-causing bacterial pathogen. Conventional methods for the detection of P. aeruginosa are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against P. aeruginosa (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 10⁴ CFU/mL and the linear range of detection was 3.13 × 10⁴-1.0 × 10⁶ CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreo

SUBMITTER: Zeng L 

PROVIDER: S-EPMC7985707 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature