Intra-Laboratory Validation of Alpha-Galactosidase Activity Measurement in Dietary Supplements.

ABSTRACT:

Introduction

Alpha-galactosidase (α-Gal) is an enzyme responsible for the hydrolyzation of glycolipids and glycoprotein commonly found in dietary sources. More than 20% of the general population suffers from abdominal pain or discomfort caused by intestinal gas and by indigested or partially digested food residuals. Therefore, α-Gal is used in dietary supplements to reduce intestinal gases and help complex food digestion. Marketed enzyme-containing dietary supplements must be produced in accordance with the Food and Drug Administration (FDA) regulations for Current Good Manufacturing Practice (cGMPs).

Aim

in this work we illustrated the process used to develop and validate a spectrophotometric enzymatic assay for α-Gal activity quantification in dietary supplements.

Metho

SUBMITTER: Fabris E 

PROVIDER: S-EPMC8000382 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature