NMR visibility of deuterium-labeled liver glycogen in vivo.

ABSTRACT:

Purpose

Deuterium metabolic imaging (DMI) combined with [6,6'-² H₂ ]-glucose has the potential to detect glycogen synthesis in the liver. However, the similar chemical shifts of [6,6'-² H₂ ]-glucose and [6,6'-² H₂ ]-glycogen in the ² H NMR spectrum make unambiguous detection and separation difficult in vivo, in contrast to comparable approaches using ¹³ C MRS. Here the NMR visibility of ² H-labeled glycogen is investigated to better understand its potential contribution to the observed signal in liver following administration of [6,6'-² H₂ ]-glucose.

Methods

Mice were provided drinking water containing ² H-labeled glucose. High-resolution NMR an

SUBMITTER: De Feyter HM 

PROVIDER: S-EPMC8005460 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature