Head-to-head validation of six immunoassays for SARS-CoV-2 in hospitalized patients.

ABSTRACT:

Background

Detecting SARS-CoV-2 antibodies may help to diagnose COVID-19. Head-to-head validation of different types of immunoassays in well-characterized cohorts of hospitalized patients remains needed.

Methods

We validated three chemiluminescence immunoassays (CLIAs) (Liaison, Elecsys, and Abbott) and one single molecule array assay (SIMOA) (Quanterix) for automated analyzers, one rapid immunoassay RIA (AllTest), and one ELISA (Wantai) in parallel in first samples from 126 PCR confirmed COVID-19 hospitalized patients and 158 pre-COVID-19 patients. Specificity of the AllTest was also tested in 106 patients with confirmed parasitic and dengue virus infections. Specificity of the Wantai assay was not tested due to limitations in sample volumes.

Results

Overall sensitiv

SUBMITTER: Zonneveld R 

PROVIDER: S-EPMC8053367 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature