Regulation of translation by methylation multiplicity of 18S rRNA.

ABSTRACT: N⁶-methyladenosine (m⁶A) is a conserved ribonucleoside modification that regulates many facets of RNA metabolism. Using quantitative mass spectrometry, we find that the universally conserved tandem adenosines at the 3' end of 18S rRNA, thought to be constitutively di-methylated (m⁶₂A), are also mono-methylated (m⁶A). Although present at substoichiometric amounts, m⁶A at these positions increases significantly in response to sulfur starvation in yeast cells and mammalian cell lines. Combining yeast genetics and ribosome profiling, we provide evidence to suggest that m⁶A-bearing ribosomes carry out translation distinctly from m⁶₂A-bearing ribosomes, featuring a striking specificity for sulfur m

SUBMITTER: Liu K 

PROVIDER: S-EPMC8063911 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature