Hydrogen sulfide treatment at the late growth stage of Saccharomyces cerevisiae extends chronological lifespan.

ABSTRACT: Previous studies demonstrated that lifelong treatment with a slow H₂S releasing donor extends yeast chronological lifespan (CLS), but it is not clear when the action of H₂S benefits to CLS during yeast growth. Here, we show that short H₂S treatments by using NaHS as a fast H₂S releasing donor at 96 hours after inoculation extended yeast CLS while NaHS treatments earlier than 72 hours after inoculation failed to do so. To reveal the mechanism, we analyzed the transcriptome of yeast cells with or without the early and late NaHS treatments. We found that both treatments had similar effects on pathways related to CLS regulation. Follow-up qPCR and ROS analyses suggest that altered expression of some antioxidant genes by the early NaHS treatments were

SUBMITTER: Shah AA 

PROVIDER: S-EPMC8064171 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature