Identification of Vitamin K3 and its analogues as covalent inhibitors of SARS-CoV-2 3CL^pro.

ABSTRACT: After the emergence of the pandemic, repurposed drugs have been considered as a quicker way of finding potential antiviral agents. SARS-CoV-2 3CL^pro is essential for processing the viral polyproteins into mature non-structural proteins, making it an attractive target for developing antiviral agents. Here we show that Vitamin K3 screened from the FDA-Approved Drug Library containing an array of 1,018 compounds has potent inhibitory activity against SARS-CoV-2 3CL^pro with the IC₅₀ value of 4.78 ± 1.03 μM, rather than Vitamin K1, K2 and K4. Next, the time-dependent inhibitory experiment was carried out to confirm that Vitamin K3 could form the covalent bond with SARS-CoV-2 3CL^pro. Then we analyzed the structure-activity relationship of Vitamin K3 an

SUBMITTER: Wang R 

PROVIDER: S-EPMC8064871 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature