Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements.

ABSTRACT:

Background

Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibility of metagenomics-based microbiome profiling of human fecal samples.

Results

In the first phase, we performed a head-to-head comparison of a wide range of protocols for DNA extraction and sequencing library construction using defined mock communities, to identify performant protocols and pinpoint sources of inaccuracy in quantification. In the second phase, we validated performant protocols with respect to their variability of measurement results within a single laboratory (that

SUBMITTER: Tourlousse DM 

PROVIDER: S-EPMC8082873 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature