The regulatory genome of the malaria vector Anopheles gambiae: integrating chromatin accessibility and gene expression.

ABSTRACT: Anopheles gambiae mosquitoes are primary human malaria vectors, but we know very little about their mechanisms of transcriptional regulation. We profiled chromatin accessibility by the assay for transposase-accessible chromatin by sequencing (ATAC-seq) in laboratory-reared A. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data, we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue-specific gene expression and to predict the in vivo binding sites of re

SUBMITTER: Ruiz JL 

PROVIDER: S-EPMC8092447 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature