Transcranial chronic optical access to longitudinally measure cerebral blood flow.

ABSTRACT:

Background

The regulation of cerebral blood flow is critical for normal brain functioning, and many physiological and pathological conditions can have long-term impacts on cerebral blood flow. However, minimally invasive tools to study chronic changes in animal models are limited.

New method

We developed a minimally invasive surgical technique (cyanoacrylate skull, CAS) allowing us to image cerebral blood flow longitudinally through the intact mouse skull using laser speckle imaging.

Results

With CAS we were able to detect acute changes in cerebral blood flow induced by hypercapnic challenge. We were also able to image cerebral blood flow dynamics with laser speckle imaging for over 100 days. Furthermore, the relative cerebral blood flow remained stable in mice from 3

SUBMITTER: Hoover EM 

PROVIDER: S-EPMC8095351 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature