Superoxide free radical spin-lattice relaxivity: A quench-assisted MR study.

ABSTRACT:

Purpose

QuEnch-assiSTed (QUEST) MRI provides a unique biomarker of excessive production of paramagnetic free radicals (oxidative stress) in vivo. The contribution from superoxide, a common upstream species found in oxidative stress-based disease, to the QUEST metric is unclear. Here, we begin to address this question by measuring superoxide spin-lattice relaxivity (r1) in phantoms.

Methods

Stable superoxide free radicals were generated in water phantoms of potassium superoxide ( KO2) . To measure r1, 1/T₁ of different concentration solutions of KO₂ in the presence and absence of the antioxidant superoxide dismutase were measured. The 1/T₁ confounding factors including acquisition sequence, pH, and water source were also evaluated.

Results<

SUBMITTER: MacKinnon MJ 

PROVIDER: S-EPMC8113059 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature