Evaluation of [¹⁸F]F-DPA as a target for TSPO in head and neck cancer under normal conditions and after radiotherapy.

ABSTRACT:

Background

Many malignant tumours have increased TSPO expression, which has been related to a poor prognosis. TSPO-PET tracers have not comprehensively been evaluated in peripherally located tumours. This study aimed to evaluate whether N,N-diethyl-2-(2-(4-([¹⁸F]fluoro)phenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide ([¹⁸F]F-DPA) can reflect radiotherapy (RT)-induced changes in TSPO activity in head and neck squamous cell carcinoma (HNSCC).

Methods

RT was used to induce inflammatory responses in HNSCC xenografts and cells. [¹⁸F]F-DPA uptake was measured in vivo in non-irradiated and irradiated tumours, followed by ex vivo biodistribution, autoradiography, and radiometabolite analysis. In vitro studies were performed in parental and

SUBMITTER: Tuominen S 

PROVIDER: S-EPMC8113193 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature