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Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer's disease.

ABSTRACT: Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease. We recently developed scREAD, a database to provide comprehensive analyses of all the existing AD scRNA-seq and snRNA-seq data from the public domain. Here, we report protocols for using the scREAD web interface and running the backend workflow locally. Our protocols enable custom analyses of AD single-cell and single-nucleus gene expression profiles. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2020).

SUBMITTER: Wang C

PROVIDER: S-EPMC8113978 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer's disease.

Wang Cankun C Xiang Yujia Y Fu Hongjun H Ma Qin Q

STAR protocols 20210503 2

Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease. We recently developed scREAD, a database to provide comprehensive analyses of all the existing AD scRNA-seq and snRNA-seq data from the public domain. Here, we report protocols for using the scREAD web interface and running the backend workflow locally. Our protocols enable custom analyses of AD single ...[more]

PMID: 34013209

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