The CD39⁺ HBV surface protein-targeted CAR-T and personalized tumor-reactive CD8⁺ T cells exhibit potent anti-HCC activity.

ABSTRACT: CD39, expressed by tumor-infiltrating lymphocytes (TILs), is a marker to identify tumor-reactive T cells, which is frequently associated with stronger antitumor activity than bystander T cells in a variety of malignancies. Therefore, CD39 could be a promising marker for identifying the active antitumor immune cells used for cellular immunotherapy. To test this possibility, we constructed the hepatitis B virus (HBV) surface protein-specific chimeric antigen receptor T cells (HBVs-CAR-T cells) and generated the personalized tumor-reactive CD8⁺ T cells. We subsequently assessed their antitumor efficiency mainly with a co-culture system for autologous HBVs⁺ HCC organoid and T cells. We found that both CD39⁺ HBVs-CAR-T and CD39⁺ personalized tumor-rea

SUBMITTER: Zou F 

PROVIDER: S-EPMC8116602 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature