Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit α₂δ-1 but not α₂δ-3.

ABSTRACT: N-type voltage-gated calcium channels (Ca_V2.2) are predominantly expressed at presynaptic terminals, and their function is regulated by auxiliary α₂δ and β subunits. All four mammalian α₂δ subunits enhance calcium currents through Ca_V1 and Ca_V2 channels, and this increase is attributed, in part, to increased Ca_V expression at the plasma membrane. In the present study we provide evidence that α₂δ-1, like α₂δ-2, is recycled to the plasma membrane through a Rab11a-dependent endosomal recycling pathway. Using a dominant-negative Rab11a mutant, Rab11a(S25N), we show that α₂δ-1 increases plasma membrane Ca_V2.2 expression by increasing the rate and extent of net forward Ca_V2.2

SUBMITTER: Meyer JO 

PROVIDER: S-EPMC8119971 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature