Trans-spliced mRNA products produced from circRNA expression vectors.
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ABSTRACT:
SUBMITTER: Chu J
PROVIDER: S-EPMC8127989 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Publications
<i>Trans</i>-spliced mRNA products produced from circRNA expression vectors.
Chu Jennifer J Robert Francis F Pelletier Jerry J
RNA (New York, N.Y.) 20210324 6
Circular (circ) RNA expression vectors are used as a method of identifying and characterizing RNA sequences that harbor internal ribosome entry site (IRES) activity. During the course of developing a vector series tailored for IRES discovery, we found evidence for the occurrence of <i>trans</i>-spliced mRNAs arising when sequences with promoter activity were embedded between the upstream CTD and downstream NTD exons of the pre-mRNA. These <i>trans</i>-spliced products regenerate the same open re ...[more]
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