Quantifying the effects of long-range ¹³C-¹³C dipolar coupling on measured relaxation rates in RNA.

ABSTRACT: Selective stable isotope labeling has transformed structural and dynamics analysis of RNA by NMR spectroscopy. These methods can remove ¹³C-¹³C dipolar couplings that complicate ¹³C relaxation analyses. While these phenomena are well documented for sites with adjacent ¹³C nuclei (e.g. ribose C1'), less is known about so-called isolated sites (e.g. adenosine C2). To investigate and quantify the effects of long-range (> 2 Å) ¹³C-¹³C dipolar interactions on RNA dynamics, we simulated adenosine C2 relaxation rates in uniformly [U-¹³C/¹⁵N]-ATP or selectively [2-¹³C]-ATP labeled RNAs. Our simulations predict non-negligible ¹³C-¹³C dipolar contributions from adenosine C4, C5, a

SUBMITTER: Olenginski LT 

PROVIDER: S-EPMC8131303 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature