Tissue-specific transcriptome profiling of the Arabidopsis inflorescence stem reveals local cellular signatures.

ABSTRACT: Genome-wide gene expression maps with a high spatial resolution have substantially accelerated plant molecular science. However, the number of characterized tissues and growth stages is still small due to the limited accessibility of most tissues for protoplast isolation. Here, we provide gene expression profiles of the mature inflorescence stem of Arabidopsis thaliana covering a comprehensive set of distinct tissues. By combining fluorescence-activated nucleus sorting and laser-capture microdissection with next-generation RNA sequencing, we characterized the transcriptomes of xylem vessels, fibers, the proximal and distal cambium, phloem, phloem cap, pith, starch sheath, and epidermis cells. Our analyses classified more than 15,000 genes as being differentially expressed among different s

SUBMITTER: Shi D 

PROVIDER: S-EPMC8136906 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature