Unknown

Dataset Information

0

Midkine-a Regulates the Formation of a Fibrotic Scar During Zebrafish Heart Regeneration.


ABSTRACT: Unlike the hearts of mammals, the adult zebrafish heart regenerates after injury. Heart cryoinjury in zebrafish triggers the formation of a fibrotic scar that gradually degrades, leading to regeneration. Midkine-a (Mdka) is a multifunctional cytokine that is activated after cardiac injury. Here, we investigated the role of mdka in zebrafish heart regeneration. We show that mdka expression was induced at 1-day post-cryoinjury (dpci) throughout the epicardial layer, whereas by 7 dpci expression had become restricted to the epicardial cells covering the injured area. To study the role of mdka in heart regeneration, we generated mdka-knock out (KO) zebrafish strains. Analysis of injured hearts showed that loss of mdka decreased endothelial cell proliferation and resulted in an arrest in heart regeneration characterized by retention of a collagenous scar. Transcriptional analysis revealed increases in collagen transcription and intense TGFβ signaling activity. These results reveal a critical role for mdka in fibrosis regulation during heart regeneration.

SUBMITTER: Grivas D 

PROVIDER: S-EPMC8138450 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

altmetric image

Publications

Midkine-a Regulates the Formation of a Fibrotic Scar During Zebrafish Heart Regeneration.

Grivas Dimitrios D   González-Rajal Álvaro Á   de la Pompa José Luis JL  

Frontiers in cell and developmental biology 20210507


Unlike the hearts of mammals, the adult zebrafish heart regenerates after injury. Heart cryoinjury in zebrafish triggers the formation of a fibrotic scar that gradually degrades, leading to regeneration. Midkine-a (Mdka) is a multifunctional cytokine that is activated after cardiac injury. Here, we investigated the role of <i>mdka</i> in zebrafish heart regeneration. We show that <i>mdka</i> expression was induced at 1-day post-cryoinjury (dpci) throughout the epicardial layer, whereas by 7 dpci  ...[more]

Similar Datasets

2021-05-26 | GSE166732 | GEO
| PRJNA701763 | ENA
| S-EPMC6992796 | biostudies-literature
2019-12-18 | GSE100029 | GEO
2019-12-18 | GSE126772 | GEO
| S-EPMC3910613 | biostudies-other
| S-EPMC7197712 | biostudies-literature
| S-EPMC3190967 | biostudies-literature
| PRJNA523576 | ENA
| S-EPMC9367779 | biostudies-literature