Systems analysis of human T helper17 cell differentiation uncovers distinct time-regulated transcriptional modules.

ABSTRACT: T helper (Th) 17 cells protect from infections and are pathogenic in autoimmunity. While human Th17 cell differentiation has been defined, the global and stepwise transcriptional changes accompanying this process remain uncharacterized. Herein, by performing transcriptome analysis of human Th17 cells, we uncovered three time-regulated modules: early, involving exclusively "signaling pathways" genes; late, characterized by response to infections; and persistent, involving effector immune functions. To assign them an inflammatory or regulatory potential, we compared Th17 cells differentiated in presence or absence of interleukin (IL)-1β, respectively. Most inflammatory genes belong to the persistent module, whereas regulatory genes are lately or persistently induced. Among inflammatory genes

SUBMITTER: Capone A 

PROVIDER: S-EPMC8138776 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature