MTORC1 stimulates cell growth through SAM synthesis and m⁶A mRNA-dependent control of protein synthesis.

ABSTRACT: The mechanistic target of rapamycin complex 1 (mTORC1) regulates metabolism and cell growth in response to nutrient, growth, and oncogenic signals. We found that mTORC1 stimulates the synthesis of the major methyl donor, S-adenosylmethionine (SAM), through the control of methionine adenosyltransferase 2 alpha (MAT2A) expression. The transcription factor c-MYC, downstream of mTORC1, directly binds to intron 1 of MAT2A and promotes its expression. Furthermore, mTORC1 increases the protein abundance of Wilms' tumor 1-associating protein (WTAP), the positive regulatory subunit of the human N⁶-methyladenosine (m⁶A) RNA methyltransferase complex. Through the control of MAT2A and WTAP levels, mTORC1 signaling stimulates m⁶A RNA modification to promote protein synt

SUBMITTER: Villa E 

PROVIDER: S-EPMC8141029 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature