Mutational signature in colorectal cancer caused by genotoxic pks⁺ E. coli.

ABSTRACT: Various species of the intestinal microbiota have been associated with the development of colorectal cancer^1,2, but it has not been demonstrated that bacteria have a direct role in the occurrence of oncogenic mutations. Escherichia coli can carry the pathogenicity island pks, which encodes a set of enzymes that synthesize colibactin³. This compound is believed to alkylate DNA on adenine residues^4,5 and induces double-strand breaks in cultured cells³. Here we expose human intestinal organoids to genotoxic pks⁺ E. coli by repeated luminal injection over five months. Whole-genome sequencing of clonal organoids before and after this exposure revealed a distinct mutational signature that was absent from organoids injected with isogenic pks-

SUBMITTER: Pleguezuelos-Manzano C 

PROVIDER: S-EPMC8142898 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature