Process analysis of pluripotent stem cell differentiation to megakaryocytes to make platelets applying European GMP.

ABSTRACT: Quality, traceability and reproducibility are crucial factors in the reliable manufacture of cellular therapeutics, as part of the overall framework of Good Manufacturing Practice (GMP). As more and more cellular therapeutics progress towards the clinic and research protocols are adapted to comply with GMP standards, guidelines for safe and efficient adaptation have become increasingly relevant. In this paper, we describe the process analysis of megakaryocyte manufacture from induced pluripotent stem cells with a view to manufacturing in vitro platelets to European GMP for transfusion. This process analysis has allowed us an overview of the entire manufacturing process, enabling us to pinpoint the cause and severity of critical risks. Risk mitigations were then proposed for each risk, desi

SUBMITTER: Lawrence M 

PROVIDER: S-EPMC8155004 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature