Adaptive laboratory evolution of β-caryophyllene producing Saccharomyces cerevisiae.

ABSTRACT:

Background

β-Caryophyllene is a plant terpenoid with therapeutic and biofuel properties. Production of terpenoids through microbial cells is a potentially sustainable alternative for production. Adaptive laboratory evolution is a complementary technique to metabolic engineering for strain improvement, if the product-of-interest is coupled with growth. Here we use a combination of pathway engineering and adaptive laboratory evolution to improve the production of β-caryophyllene, an extracellular product, by leveraging the antioxidant potential of the compound.

Results

Using oxidative stress as selective pressure, we developed an adaptive laboratory evolution that worked to evolve an engineered β-caryophyllene producing yeast strain for improved production within a few generat

SUBMITTER: Godara A 

PROVIDER: S-EPMC8157465 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature