Unknown

Dataset Information

0

Automated annotation and visualisation of high-resolution spatial proteomic mass spectrometry imaging data using HIT-MAP.


ABSTRACT: Spatial proteomics has the potential to significantly advance our understanding of biology, physiology and medicine. Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is a powerful tool in the spatial proteomics field, enabling direct detection and registration of protein abundance and distribution across tissues. MALDI-MSI preserves spatial distribution and histology allowing unbiased analysis of complex, heterogeneous tissues. However, MALDI-MSI faces the challenge of simultaneous peptide quantification and identification. To overcome this, we develop and validate HIT-MAP (High-resolution Informatics Toolbox in MALDI-MSI Proteomics), an open-source bioinformatics workflow using peptide mass fingerprint analysis and a dual scoring system to computationally assign peptide and protein annotations to high mass resolution MSI datasets and generate customisable spatial distribution maps. HIT-MAP will be a valuable resource for the spatial proteomics community for analysing newly generated and retrospective datasets, enabling robust peptide and protein annotation and visualisation in a wide array of normal and disease contexts.

SUBMITTER: Guo G 

PROVIDER: S-EPMC8163805 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8491408 | biostudies-literature
| S-EPMC6946663 | biostudies-literature
| S-EPMC4542666 | biostudies-literature
| S-EPMC5080735 | biostudies-literature
| S-EPMC7186543 | biostudies-literature
| S-EPMC9719826 | biostudies-literature
| S-EPMC5693761 | biostudies-literature
| S-EPMC7806579 | biostudies-literature
| S-EPMC8590398 | biostudies-literature
| S-EPMC3690593 | biostudies-literature