Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium.

ABSTRACT: This study aimed to investigate the effects of cofD gene knock-out on the synthesis of coenzyme F₄₂₀ and production of methane in Methanobrevibacter ruminantium (M. ruminantium). The experiment successfully constructed a cofD gene knock-out M. ruminantium via homologous recombination technology. The results showed that the logarithmic phase of mutant M. ruminantium (12 h) was lower than the wild-type (24 h). The maximum biomass and specific growth rate of mutant M. ruminantium were significantly lower (P < 0.05) than those of wild-type, and the maximum biomass of mutant M. ruminantium was approximately half of the wild-type; meanwhile, the proliferation was reduced. The synthesis amount of coenzyme F₄₂₀ of M. ruminantium was significantly decreased (P < 0.05) after th

SUBMITTER: Ma J 

PROVIDER: S-EPMC8163928 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature