Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

ABSTRACT: Mechanical forces are conducted through myofibers and into nuclei to regulate muscle development, hypertrophy, and homeostasis. We hypothesized that nuclei in aged muscle have changes in the nuclear envelope and associated proteins, resulting in altered markers of mechano-signaling.

Methods

YAP/TAZ protein expression and gene expression of downstream targets, Ankrd1 and Cyr61, were evaluated as mechanotransduction indicators. Expression of proteins in the nuclear lamina and the nuclear pore complex (NPC) were assessed, and nuclear morphology was characterized by electron microscopy. Nuclear envelope permeability was assessed by uptake of 70 kDa fluorescent dextran.

Results

Nuclear changes with aging included a relative decrease of lamin β1 and Nup107, and a relative increase

SUBMITTER: Iyer SR 

PROVIDER: S-EPMC8165001 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature