A New Parallel High-Pressure Packing System Enables Rapid Multiplexed Production of Capillary Columns.

ABSTRACT: Reversed-phase HPLC is the most commonly applied peptide-separation technique in MS-based proteomics. Particle-packed capillary columns are predominantly used in nanoflow HPLC systems. Despite being the broadly applied standard for many years, capillary columns are still expensive and suffer from short lifetimes, particularly in combination with ultra-high-pressure chromatography systems. For this reason, and to achieve maximum performance, many laboratories produce their own in-house packed columns. This typically requires a considerable amount of time and trained personnel. Here, we present a new packing system for capillary columns enabling rapid, multiplexed column packing with pressures reaching up to 3000 bar. Requiring only a conventional gas pressure supply and methanol as the driv

SUBMITTER: Muller-Reif JB 

PROVIDER: S-EPMC8165429 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature