Using Heavy Mass Isotopomers for Protein Turnover in Heavy Water Metabolic Labeling.

ABSTRACT: Metabolic labeling followed by LC-MS-based proteomics is a powerful tool to study proteome dynamics in high-throughput experiments both in vivo and in vitro. High mass resolution and accuracy allow differentiation in isotope profiles and the quantification of partially labeled peptide species. Metabolic labeling duration introduces a time domain in which the gradual incorporation of labeled isotopes is recorded. Different stable isotopes are used for labeling. Labeling with heavy water has advantages because it is cost-effective and easy to use. The protein degradation rate constant has been modeled using exponential decay models for the relative abundances of mass isotopomers. The recently developed closed-form equations were applied to study the analytic behavior of the hea

SUBMITTER: Sadygov RG 

PROVIDER: S-EPMC8167932 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature