Structure of human factor VIIa-soluble tissue factor with calcium, magnesium and rubidium.

ABSTRACT: Coagulation factor VIIa (FVIIa) consists of a γ-carboxyglutamic acid (GLA) domain, two epidermal growth factor-like (EGF) domains and a protease domain. FVIIa binds three Mg²⁺ ions and four Ca²⁺ ions in the GLA domain, one Ca²⁺ ion in the EGF1 domain and one Ca²⁺ ion in the protease domain. Further, FVIIa contains an Na⁺ site in the protease domain. Since Na⁺ and water share the same number of electrons, Na⁺ sites in proteins are difficult to distinguish from waters in X-ray structures. Here, to verify the Na⁺ site in FVIIa, the structure of the FVIIa-soluble tissue factor (TF) complex was solved at 1.8 Å resolution containing Mg²⁺, Ca²⁺ and Rb⁺ ions. In this structure,

SUBMITTER: Vadivel K 

PROVIDER: S-EPMC8171065 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature