Applications and advancements of FT-ICR-MS for interactome studies.

ABSTRACT: The set of all intra- and intermolecular interactions, collectively known as the interactome, is currently an unmet challenge for any analytical method, but if measured, could provide unparalleled insight on molecular function in living systems. Developments and applications of chemical cross-linking and high-performance mass spectrometry technologies are beginning to reveal details on how proteins interact in cells and how protein conformations and interactions inside cells change with phenotype or during drug treatment or other perturbations. A major contributor to these advances is Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) technology and its implementation with accurate mass measurements on cross-linked peptide-pair precursor and fragment ions to enable imp

SUBMITTER: Chavez JD 

PROVIDER: S-EPMC8184889 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature