Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis.

ABSTRACT:

Background

Patients with chronic obstructive pulmonary disease (COPD) frequently suffer from chronic bronchitis (CB) and display steroid-resistant inflammation with increased sputum neutrophils and macrophages. Recently, a causal link between mucus hyper-concentration and disease progression of CB has been suggested.

Methods

In this study, we have evaluated the steroid sensitivity of purified, patient-derived sputum and alveolar macrophages and used a novel mechanistic cross-talk assay to examine how macrophages and bronchial epithelial cells cross-talk to regulate MUC5B production.

Results

We demonstrate that sputum plug macrophages isolated from COPD patients with chronic bronchitis (COPD/CB) are chronically activated and only partially respond to ex vivo corticoste

SUBMITTER: Andelid K 

PROVIDER: S-EPMC8186034 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature