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Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber.

ABSTRACT: Cells typically exist in a highly dynamic environment, which cannot easily be recreated in culture dishes or microwell plates. Microfluidic devices can provide precise control of the time, dose, and orientation of a stimulus, while simultaneously capturing quantitative single-cell data. The approach is particularly powerful when combined with the genetically tractable yeast model organism. The GPCR pathway in yeast is structurally conserved and functionally interchangeable with those in humans. We describe the implementation of a microfluidic device to investigate morphological and transcriptional responses of yeast to a gradient or pulse administration of a GPCR ligand, the peptide mating pheromone α-factor.

SUBMITTER: Suzuki SK

PROVIDER: S-EPMC8188611 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber.

Suzuki Sara Kimiko SK Kelley Joshua B JB Elston Timothy C TC Dohlman Henrik G HG

Methods in molecular biology (Clifton, N.J.) 20210101

Cells typically exist in a highly dynamic environment, which cannot easily be recreated in culture dishes or microwell plates. Microfluidic devices can provide precise control of the time, dose, and orientation of a stimulus, while simultaneously capturing quantitative single-cell data. The approach is particularly powerful when combined with the genetically tractable yeast model organism. The GPCR pathway in yeast is structurally conserved and functionally interchangeable with those in humans. ...[more]

PMID: 34085275

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