FRET-Based Genetically Encoded Sensor to Monitor Silver Ions.

ABSTRACT: Silver is commonly used in wound dressing, photography, health care products, laboratories, pharmacy, biomedical devices, and several industrial purposes. Silver (Ag⁺) ions are more toxic pollutants widely scattered in the open environment by natural processes and dispersed in soil, air, and water bodies. Ag⁺ binds with metallothionein, macroglobulins, and albumins, which may lead to the alteration of various enzymatic metabolic pathways. To analyze the uptake and metabolism of silver ions in vitro as well as in cells, a range of high-affinity fluorescence-based nanosensors has been constructed using a periplasmic protein CusF, a part of the CusCFBA efflux complex, which is involved in providing resistance against copper and silver ions in Escherichia coli</

SUBMITTER: Agrawal N 

PROVIDER: S-EPMC8190795 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature