Protocol for screening and expression studies of T-DNA and tagging-based insertional knox mutants in Arabidopsis thaliana.

ABSTRACT: KNOTTED1-like homeobox (KNOX) genes serve important roles in meristem function and many developmental processes in all higher plants. In Arabidopsis, studies of KNOX genes especially among members of class II KNOX genes remain limited and functional data are largely lacking. In the present study, we established a reproducible protocol that is important for genetic studies of KNOX genes using Arabidopsis insertional mutants. This protocol contains a reproducible and serial procedure containing detailed and step-by-step laboratory and field works covering all experiment steps from the screening of homozygous mutant lines to the KNOX expression analysis using qRT-PCR in a single paper. The troubleshooting and challenges that might occur

SUBMITTER: Sunaryo W 

PROVIDER: S-EPMC8200336 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature