Addition of mirror-image L-DNA elements to DNA amplification circuits to distinguish leakage from target signal.

ABSTRACT: DNA amplification circuits that rely on thermodynamically-driven hybridization events triggered by a target nucleic acid are becoming increasingly utilized due to their relative simplicity. A drawback of these circuits is that non-specific amplification, or circuit leakage, must be estimated using a separate "no-target" control reaction to eliminate false positives. Aside from requiring an additional reaction, the problem with this approach is the difficulty of creating a no-target control for biological specimens. To overcome this limitation, we propose a strategy that combines both reactions into the same tube using naturally-occurring right-handed D-DNA circuit elements for the target detection reaction and identical synthetic mirror-image left-handed L-DNA circuit elements for the no-t

SUBMITTER: Zimmers ZA 

PROVIDER: S-EPMC8202133 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature