Ontology highlight
ABSTRACT:
SUBMITTER: Jiang Y
PROVIDER: S-EPMC8215978 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20210506 24
A method for high-throughput counting and superresolution mapping of surface proteins on exosomes is described. The method combines a single-molecule sensitive flow technique and an adaptive superresolution imaging method. Exosomes stained with membrane dye and dye-conjugated antibodies were analyzed using a microfluidic platform at a flow rate of 100 exosome s<sup>-1</sup> to determine size and protein copy number. Superresolution mapping was performed with exosomes labeled with novel transisto ...[more]