Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks.

ABSTRACT: Environmental DNA (eDNA) techniques refer to utilizing the organisms' DNA extracted from environment samples to genetically identify target species without capturing actual organisms. eDNA metabarcoding via high-throughput sequencing can simultaneously detect multiple fish species from a single water sample, which is a powerful tool for the qualitative detection and quantitative estimates of multiple fish species. However, sequence counts obtained from eDNA metabarcoding may be influenced by many factors, of which primer bias is one of the foremost causes of methodological error. The performance of 18 primer pairs for COI, cytb, 12S rRNA, and 16S rRNA mitochondrial genes, which are all frequently used in fish eDNA metabarcoding, were evaluated in the current stu

SUBMITTER: Shu L 

PROVIDER: S-EPMC8216916 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature