Single-molecule Study on the Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore.

ABSTRACT: Nonribosomal peptides (NRPs) are a type of secondary metabolites mostly originated from microorganisms such as bacteria and fungi. Their proteolytic stability, highly selective bioactivity, and microorganism-specificity have made them an attractive source of drugs for the pharmaceutical industry. Herein, with microcystins (MCs) as a NRP model, we, for the first time, proposed a sensitive method to study the interactions between NRPs and the protein nanopore. Due to the large molecular size (~3 nm diameter) of MCs and their net negative charges, MCs failed to translocate through the α-hemolysin (α-HL) protein channel. Our results demonstrated that the biomolecular interaction of MC-α-HL protein was significantly affected by the applied potential bias. The constant blockage amplitude in the

SUBMITTER: Zhou S 

PROVIDER: S-EPMC8221584 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature