Ontology highlight
ABSTRACT:
SUBMITTER: Kahn-Krell A
PROVIDER: S-EPMC8226172 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Kahn-Krell Asher A Pretorius Danielle D Ou Jianfa J Fast Vladimir G VG Litovsky Silvio S Berry Joel J Liu Xiaoguang Margaret XM Zhang Jianyi J
Frontiers in bioengineering and biotechnology 20210611
Human induced-pluripotent stem cells (hiPSCs) can be efficiently differentiated into cardiomyocytes (hiPSC-CMs) via the GiWi method, which uses small-molecule inhibitors of glycogen synthase kinase (GSK) and tankyrase to first activate and then suppress Wnt signaling. However, this method is typically conducted in 6-well culture plates with two-dimensional (2D) cell sheets, and consequently, cannot be easily scaled to produce the large numbers of hiPSC-CMs needed for clinical applications. Cell ...[more]