Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC8237658 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Liu Yanjun Y Zeng Ruxin R Wang Ruixuan R Weng Yicheng Y Wang Ruixiang R Zou Peng P Chen Peng R PR
Proceedings of the National Academy of Sciences of the United States of America 20210601 25
Proteome-wide profiling of protein phosphorylation has been widely used to reveal the underlying mechanism of diverse cellular signaling events. Yet, characterizing subcellular phosphoproteome with high spatial-temporal resolution has remained challenging. Herein, we developed a subcellular-specific uncaging-assisted biotinylation and mapping of phosphoproteome (SubMAPP) strategy to monitor the phosphorylation dynamics of subcellular proteome in living cells and animals. Our method capitalizes o ...[more]