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Expansion microscopy-based imaging of nuclear structures in cultured cells.

ABSTRACT: Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion of U2OS cells with concurrent DNA staining. A custom 3D-printed gel cutter and chambered slides minimize gel drift, facilitating analysis of the components of nuclear structures at nanoscale resolution by conventional microscopy or Airyscan confocal imaging. For complete information on the generation and use of this protocol, please refer to Do et al. (2020).

SUBMITTER: Gaudreau-Lapierre A

PROVIDER: S-EPMC8243706 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Expansion microscopy-based imaging of nuclear structures in cultured cells.

Gaudreau-Lapierre Antoine A Mulatz Kirk K Béïque Jean-Claude JC Trinkle-Mulcahy Laura L

STAR protocols 20210626 3

Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion of U2OS cells with concurrent DNA staining. A custom 3D-printed gel cutter and chambered slides minimize gel drift, facilitating analysis of the components of nuclear structures at nanoscale resolution by conv ...[more]

PMID: 34223201

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