Ontology highlight
ABSTRACT:
SUBMITTER: Gaudreau-Lapierre A
PROVIDER: S-EPMC8243706 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Gaudreau-Lapierre Antoine A Mulatz Kirk K Béïque Jean-Claude JC Trinkle-Mulcahy Laura L
STAR protocols 20210626 3
Expansion microscopy is a sample preparation technique in which fixed and immunostained cells or tissues are embedded in a cross-linked network of swellable polyelectrolyte hydrogel that expands isotropically upon addition of deionized water. We utilize the X10 method for tenfold expansion of U2OS cells with concurrent DNA staining. A custom 3D-printed gel cutter and chambered slides minimize gel drift, facilitating analysis of the components of nuclear structures at nanoscale resolution by conv ...[more]