Patient-derived models recapitulate heterogeneity of molecular signatures and drug response in pediatric high-grade glioma.

ABSTRACT: Pediatric high-grade glioma (pHGG) is a major contributor to cancer-related death in children. In vitro and in vivo disease models reflecting the intimate connection between developmental context and pathogenesis of pHGG are essential to advance understanding and identify therapeutic vulnerabilities. Here we report establishment of 21 patient-derived pHGG orthotopic xenograft (PDOX) models and eight matched cell lines from diverse groups of pHGG. These models recapitulate histopathology, DNA methylation signatures, mutations and gene expression patterns of the patient tumors from which they were derived, and include rare subgroups not well-represented by existing models. We deploy 16 new and existing cell lines for high-throughput screening (HTS). In vitro HTS results predict variable in v

SUBMITTER: He C 

PROVIDER: S-EPMC8253809 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature